Description
Principle of the assay: A2R1Ab ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with Angiotensin 2 receptor 1 antigen. Standards or samples are then added to the microtiter plate wells and A2R1Ab if present, will bind to the antigen pre-coated wells. In order to quantitatively determine the amount of A2R1Ab present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated Angiotensin 2 receptor 1 antigen is added to each well to "sandwich" the A2R1Ab immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain A2R1Ab and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The A2R1Ab concentration in each sample is interpolated from this standard curve